KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay kit
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CAT.: A1090008D
KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between STAT6 protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑STAT6 protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑CRBN/DDB1 protein complex. The Tag1‑STAT6 protein and the Tag2‑CRBN/DDB1 protein complex can form a ternary complex mediated by the positive reference PROTAC molecule AK‑1690, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between STAT6 and CRBN/DDB1.
standard curve
| CAT. | Description | Size |
| A1090008D | KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay kit | 100 tests |
|
• 1 vial of 5 µL PROTAC standard 1 AK-1690 (50 mM in DMSO) • 1 vial 20 μL/vial Tag1-STAT6 • 1 vial 20 μL/vial Tag2-CRBN/DDB1 • 1 vial 5 µL/vial mAb anti-Tag1 - Solar Eu • 1 vial 5 µL/vial mAb anti-Tag2 - LA • 1 vial 5 µL/vial TCEP (1 M) • 2 vials 2 mL/vial PROTAC Diluent Buffer #2 • 1 vial 2 mL/vial PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between STAT6 protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑STAT6 protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑CRBN/DDB1 protein complex. The Tag1‑STAT6 protein and the Tag2‑CRBN/DDB1 protein complex can form a ternary complex mediated by the positive reference PROTAC molecule AK‑1690, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between STAT6 and CRBN/DDB1.
standard curve
| CAT. | Description | Size |
| A1090008S | KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay kit | 500 tests |
|
• 1 vial of 10 µL PROTAC standard 1 AK-1690 (50 mM in DMSO) • 1 vial 100 μL/vial Tag1-STAT6 • 1 vial 100 μL/vial Tag2-CRBN/DDB1 • 1 vial 25 µL/vial mAb anti-Tag1 - Solar Eu • 1 vial 25 µL/vial mAb anti-Tag2 - LA • 1 vial 120 µL/vial (A1010023S) TCEP (1 M) • 1 bottle 50 mL/bottle (A1010046S) PROTAC Diluent Buffer #2 • 1 bottle 30 mL/bottle (A1010047S) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay Kit can be used to screen PROTAC molecules that mediate the formation of a ternary complex between STAT6 protein and the CRBN/DDB1 complex. This kit is a binding immunoassay developed using KeyTec® TR-FRET technology, featuring simplicity, speed, high accuracy, and excellent reproducibility.
The basic principle of this method is as shown in the figure below. The KeyTec® TR-FRET Solar Eu1‑conjugated anti‑Tag1 antibody specifically recognizes the Tag1‑STAT6 protein, while the KeyTec® TR‑FRET LA2‑conjugated anti‑Tag2 antibody specifically recognizes the Tag2‑CRBN/DDB1 protein complex. The Tag1‑STAT6 protein and the Tag2‑CRBN/DDB1 protein complex can form a ternary complex mediated by the positive reference PROTAC molecule AK‑1690, bringing Solar Eu and LA into close proximity. Upon excitation by an external light source, energy resonance transfer occurs between the donor and acceptor. Detection of signal intensity at a specific wavelength (665 nm) allows determination of the PROTAC molecule‑mediated binding between STAT6 and CRBN/DDB1.
standard curve
| CAT. | Description | Size |
| A1090008L | KeyTec® TR-FRET STAT6 / CRBN PROTAC Binding Assay kit | 5,000 tests |
|
• 1 vial of 50 µL PROTAC standard 1 AK-1690 (50 mM in DMSO) • 1 vial 1 mL/vial Tag1-STAT6 • 1 vial 1 mL/vial Tag2-CRBN/DDB1 • 1 vial 250 µL/vial mAb anti-Tag1 - Solar Eu • 1 vial 250 µL/vial mAb anti-Tag2 - LA • 2 vials 120 µL/vial (A1010023S) TCEP (1 M) • 1 bottle 200 mL/bottle (A1010046L) PROTAC Diluent Buffer #2 • 1 bottle 120 mL/bottle (A1010047L) PROTAC Detection Buffer #2 |
||
| Certificate of Analysis | Limitations | Storage Conditions |
| LOT. | For research use only |
-60℃ or below |
English
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